Anti-Natriuretic肽Receptor B抗体(ab55724)


  • 产品名称
    Anti-Natriuretic肽Receptor B抗体
    参阅全部 Natriuretic Peptide Receptor B 一抗
  • 描述
    小鼠单克隆抗体to Natriuretic肽Receptor B
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: AKNDHYRTLV RTGPSAPKLG EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH QVYAREPGGP EQATHFIRAN GRIVYICGPL , corresponding to amino acids 131-231 of Human Natriuretic Peptide Receptor B

  • 阳性对照
    • ICC/IF: SKNSH cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab55724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • 功能
    Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
  • 疾病相关
    Defects in NPR2 are the cause of acromesomelic dysplasia Maroteaux type (AMDM) [MIM:602875]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). AMDM is an autosomal recessive form characterized by axial skeletal involvement with wedging of vertebral bodies. In AMDM all skeletal elements are present but show abnormal rates of linear growth.
  • 序列相似性
    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylation of the protein kinase-like domain is required for full activation by CNP.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMDM antibody
    • ANP-B antibody
    • ANPB antibody
    • ANPR-B antibody
    • ANPRB antibody
    • ANPRB_HUMAN antibody
    • Atrial natriuretic peptide B type receptor antibody
    • Atrial natriuretic peptide receptor 2 antibody
    • Atrial natriuretic peptide receptor type B antibody
    • Atrionatriuretic peptide receptor B antibody
    • GC-B antibody
    • GCB antibody
    • Guanylate cyclase B antibody
    • GUC 2B antibody
    • GUC2B antibody
    • GUCY2B antibody
    • Natriuretic peptide receptor B antibody
    • Natriuretic peptide receptor B/guanylate cyclase B antibody
    • NPR-B antibody
    • Npr2 antibody
    • NPRB antibody
    • NPRBi antibody
    see all

Anti-Natriuretic Peptide Receptor B antibody 图像

  • ICC/IF image of ab55724 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43um..

  • Natriuretic Peptide Receptor B antibody (ab55724) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab55724 Natriuretic Peptide Receptor B antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab55724 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Natriuretic Peptide Receptor B antibody (ab55724)参考文献

ab55724 has not yet been referenced specifically in any publications.

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