概述
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产品名称Anti-Natriuretic多肽Receptor A抗体
参阅全部 Natriuretic Peptide Receptor A 一抗 -
描述兔多克隆抗体to Natriuretic多肽Receptor A
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human Natriuretic Peptide Receptor A.
(Peptide available as ab41439.)
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阳性对照
- This antibody gave a positive signal in Kidney (Human) Tissue Lysate - adult normal tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab40817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
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序列相似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain. -
翻译后修饰Phosphorylation of the protein kinase-like domain is required for full activation by ANP.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 4881 Human
- Entrez Gene: 18160 Mouse
- Entrez Gene: 24603 Rat
- Omim: 108960 Human
- SwissProt: P16066 Human
- SwissProt: P18293 Mouse
- SwissProt: P18910 Rat
- Unigene: 490330 Human
see all -
别名
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
see all
图片
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Western blot - Anti-Natriuretic Peptide Receptor A antibody (ab40817)Anti-Natriuretic Peptide Receptor A antibody (ab40817) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 140 kDa (why is the actual band size different from the predicted?)
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Natriuretic Peptide Receptor A antibody (ab40817)ICC/IF image of ab40817 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40817, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
文献
ab40817 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"