概述
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产品名称Anti-Natriuretic肽Receptor A抗体
参阅全部 Natriuretic Peptide Receptor A 一抗 -
描述兔多克隆抗体to Natriuretic肽Receptor A
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human Natriuretic Peptide Receptor A.
(Peptide available as ab41439.)
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阳性对照
- This antibody gave a positive signal in Kidney (Human) Tissue Lysate - adult normal tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab40817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
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序列相似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain. -
翻译后修饰Phosphorylation of the protein kinase-like domain is required for full activation by ANP.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 4881 Human
- Entrez Gene: 18160 Mouse
- Entrez Gene: 24603 Rat
- Omim: 108960 Human
- SwissProt: P16066 Human
- SwissProt: P18293 Mouse
- SwissProt: P18910 Rat
- Unigene: 490330 Human
see all -
别名
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
see all
Anti-Natriuretic Peptide Receptor A antibody 图像
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Western blot - Natriuretic Peptide Receptor A antibody (ab40817)Anti-Natriuretic Peptide Receptor A antibody (ab40817) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 119 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Natriuretic Peptide Receptor A antibody (ab40817)ICC/IF image of ab40817 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40817, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-Natriuretic Peptide Receptor A antibody (ab40817)参考文献
ab40817 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"