• 产品名称Anti-NAT8L抗体
    参阅全部 NAT8L 一抗
  • 描述
    兔多克隆抗体to NAT8L
  • 经测试应用适用于: ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide derived from the C terminal domain of mouse FLJ37478 protein



Our Abpromise guarantee covers the use of ab76842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/150. (see Abreview)
IHC-Fr 1/50 - 1/200.
WB 1/100 - 1/1000. Predicted molecular weight: 33 kDa.


  • 功能Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.
  • 组织特异性Expressed in brain.
  • 序列相似性Belongs to the camello family.
    Contains 1 N-acetyltransferase domain.
  • 细胞定位Cytoplasm. Membrane. Microsome membrane. Mitochondrion membrane. Rough endoplasmic reticulum membrane. Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110038O08Rik antibody
    • Camello-like protein 3 antibody
    • CML3 antibody
    • Hcml3 antibody
    • N acetyltransferase 8 like (GCN5 related, putative) antibody
    • N-acetylaspartate synthetase antibody
    • N-acetyltransferase 8-like protein antibody
    • NAA synthetase antibody
    • NAT8 like antibody
    • Nat8l antibody
    • NAT8L_HUMAN antibody
    • protein Shati antibody
    • Shati antibody
    see all

Anti-NAT8L antibody 图像

  • ab76842 staining FLJ37478 in CHO cells transfected with mouse Nat8l cDNA by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/150 in 1% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Cy2® conjugated goat anti-rabbit polyclonal, diluted 1/200.

    See Abreview

  • All lanes : Anti-NAT8L antibody (ab76842) at 1/1000 dilution (in TBS + 0.05% Tween 20 for 1 hour at 22°C)

    Lane 1 : CHO-K1 cells expressing mouse Nat8L cDNA
    Lane 2 : CHO-K1 cells transfected with empty vector

    Lysates/proteins at 70 µg per lane.

    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreveiw

    Blocking STep: 3% Milk for 30 minutes at 22 °C

    See Abreview

Anti-NAT8L antibody (ab76842)参考文献

ab76842 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for your enquiry.

This product is a rabbit whole antiserum (non-purified) product and there is an import restriction into Japan. Depending on the country of origin, Abcam can sometimes get custom clearance if we have the proper doc...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CHO-K1 cells transfected with Nat8L cDNA)
Loading amount 70 µg
Specification CHO-K1 cells transfected with Nat8L cDNA
Gel Running Conditions Reduced Denaturing (10%, polyacrylamide)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Feb 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (CHO cells transfected with mouse Nat8l cDNA)
Specification CHO cells transfected with mouse Nat8l cDNA
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 20 2011