Anti-NARS抗体[NARS2G5] (ab53473)

概述

  • 产品名称Anti-NARS抗体[NARS2G5]
    参阅全部 NARS 一抗
  • 描述
    小鼠单克隆抗体[NARS2G5] to NARS
  • 经测试应用适用于: WB, IP, ICC, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment from Human NARS.

  • 阳性对照
    • HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • 克隆单克隆
  • 克隆编号NARS2G5
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab53473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

靶标

Anti-NARS antibody [NARS2G5] 图像

  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
  • ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-NARS antibody [NARS2G5] (ab53473)参考文献

ab53473 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab53473.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"