Anti-NARS抗体[NARS2G5] (ab53473)

概述

  • 产品名称
    Anti-NARS抗体[NARS2G5]
    参阅全部 NARS 一抗
  • 描述
    小鼠单克隆抗体[NARS2G5] to NARS
  • 经测试应用
    适用于: WB, IP, ICC, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment from Human NARS.

  • 阳性对照
    • HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • 克隆
    单克隆
  • 克隆编号
    NARS2G5
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

靶标

  • 序列相似性
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3010001M15Rik antibody
    • AA960128 antibody
    • AsnRS antibody
    • ASNS antibody
    • Asparagine tRNA ligase 1, cytoplasmic antibody
    • Asparagine tRNA ligase antibody
    • Asparagine--tRNA ligase antibody
    • Asparaginyl tRNA synthetase antibody
    • Asparaginyl-tRNA synthetase antibody
    • C78150 antibody
    • cytoplasmic antibody
    • EC 6.1.1.22 antibody
    • LRRGT00113 antibody
    • MGC116236 antibody
    • Nars antibody
    • NARS1 antibody
    • NRS antibody
    • SYNC_HUMAN antibody
    see all

Anti-NARS antibody [NARS2G5] 图像

  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
  • ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-NARS antibody [NARS2G5] (ab53473)参考文献

ab53473 has not yet been referenced specifically in any publications.

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