概述

  • 产品名称Anti-NARG1抗体
    参阅全部 NARG1 一抗
  • 描述
    兔多克隆抗体to NARG1
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human) from an internal sequence.

  • 阳性对照
    • Extracts from HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
  • 组织特异性Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
  • 序列相似性Contains 8 TPR repeats.
  • 翻译后修饰Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
  • 细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5730450D16Rik antibody
    • 6330400I15 antibody
    • ASTBDN antibody
    • GA19 antibody
    • Gastric cancer antigen Ga19 antibody
    • MGC29428 antibody
    • mNAT1 antibody
    • N alpha acetyltransferase 15, NatA auxiliary subunit antibody
    • N terminal acetyltransferase antibody
    • N(alpha) acetyltransferase 15, NatA auxiliary subunit antibody
    • N-alpha-acetyltransferase 15 antibody
    • N-terminal acetyltransferase antibody
    • Naa15 antibody
    • NAA15_HUMAN antibody
    • NatA auxiliary subunit antibody
    • NATH antibody
    • NMDA receptor regulated 1 antibody
    • NMDA receptor regulated protein 1 antibody
    • NMDA receptor-regulated protein 1 antibody
    • Protein tubedown 1 antibody
    • Protein tubedown-1 antibody
    • Tbdn 1 antibody
    • Tbdn100 antibody
    • Transcriptional coactivator tubedown 100 antibody
    • tubedown-1 antibody
    see all

Anti-NARG1 antibody 图像

  • All lanes : Anti-NARG1 antibody (ab65107) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • ICC/IF image of ab65107 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65107, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NARG1 antibody (ab65107)参考文献

ab65107 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65107.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"