概述

  • 产品名称Anti-NAP1L1抗体
    参阅全部 NAP1L1 一抗
  • 描述
    兔多克隆抗体to NAP1L1
  • 经测试应用适用于: IP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
    预测可用于: Rat, Chicken, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L1.

    (Peptide available as ab22418.)

  • 阳性对照
    • U2OS whole cell lysate HeLa whole cell lysate A431 whole cell lysate HEK293 whole cell lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human NAP1L1 peptide (ab22418).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the nucleosome assembly protein (NAP) family.
  • 结构域The acidic domains are probably involved in the interaction with histones.
  • 翻译后修饰Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
  • 细胞定位Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ16112 antibody
    • hNRP antibody
    • HSP22 like protein interacting protein antibody
    • MGC23410 antibody
    • MGC8688 antibody
    • NAP 1 antibody
    • NAP 1 related protein antibody
    • NAP 1L antibody
    • NAP-1-related protein antibody
    • NAP1 antibody
    • NAP1 L1 antibody
    • NAP1 related protein antibody
    • NAP1L antibody
    • Nap1l1 antibody
    • NAP1L1 protein antibody
    • NP1L1_HUMAN antibody
    • NRP antibody
    • Nucleosome assembly protein 1 like 1 antibody
    • Nucleosome assembly protein 1-like 1 antibody
    • Nucleosome assembly protein I-related protein antibody
    see all

Anti-NAP1L1 antibody 图像

  • Lanes 1 - 2 : Anti-NAP1L1 antibody (ab33076) at 1/1000 dilution
    Lanes 3 - 4 : Anti-NAP1L1 antibody (ab33076) at 1/500 dilution
    Lanes 5 - 6 : Anti-NAP1L1 antibody (ab33076) at 1/250 dilution

    Lane 1 : U2OS whole cell lysate
    Lane 2 : U2OS whole cell lysate treated with ionizing radiation
    Lane 3 : U2OS whole cell lysate
    Lane 4 : U2OS whole cell lysate treated with ionizing radiation
    Lane 5 : U2OS whole cell lysate
    Lane 6 : U2OS whole cell lysate treated with ionizing radiation


    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa (possible cleavage fragment,cross reactivity),64 kDa (possible cross reactivity).
  • All lanes : Anti-NAP1L1 antibody (ab33076) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HEK293 whole cell lysate
    Lane 4 : HeLa whole cell lysate with Human NAP1L1 peptide (ab22418) at 1 µg/ml
    Lane 5 : A431 whole cell lysate with Human NAP1L1 peptide (ab22418) at 1 µg/ml
    Lane 6 : HEK293 whole cell lysate with Human NAP1L1 peptide (ab22418) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa fluor goat polyclonal to rabbit IgG (700) at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa (possible cleavage fragment,cross reactivity),75 kDa (possible cross reactivity).
  • All lanes : Anti-NAP1L1 antibody (ab33076) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 74 kDa (possible cross reactivity).
  • NAP1L1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to NAP1L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33076.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa: NAP1L1; 49 kDa (possible cleavage fragment,cross reactivity.
  • IHC image of ab33076 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33076, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab33076 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33076, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NAP1L1 antibody (ab33076)参考文献

This product has been referenced in:
  • Cho I  et al. ATP-dependent chromatin remodeling by Cockayne syndrome protein B and NAP1-like histone chaperones is required for efficient transcription-coupled DNA repair. PLoS Genet 9:e1003407 (2013). IP ; Human . Read more (PubMed: 23637612) »
  • Ratnakumar K  et al. ATRX-mediated chromatin association of histone variant macroH2A1 regulates a-globin expression. Genes Dev 26:433-8 (2012). WB ; Human . Read more (PubMed: 22391447) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.
After blasting the immunogen sequence for ab33076, I can confirm that NAP1L4 shows no homology according to the Swiss prot blast.
Therefore it is very un-likely that the antibody cross-reacts with NAP1L4, but this ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Xenopus laevis Cell lysate - other (egg)
Total protein in input 625 µg
Specification egg
Immuno-precipitation step Other - Dynabeads
Username

Abcam user community

Verified customer

提交于 Feb 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Huh7-Lunet Cells)
Total protein in input 400 µg
Specification Huh7-Lunet Cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Feb 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Egg)
Loading amount 25 µg
Specification Egg
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 10 µg
Specification fibroblast
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"