Anti-Nanos Homologue 1 (NANOS1)抗体(ab83417)

概述

  • 产品名称
    Anti-Nanos Homologue 1 (NANOS1)抗体
    参阅全部 Nanos Homologue 1 (NANOS1) 一抗
  • 描述
    兔多克隆抗体to Nanos Homologue 1 (NANOS1)
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Pig
  • 免疫原

    Synthetic peptide: DDDDDSDEPG SRGRYLGSAL ELRALELCAG PAEAGLLEER FAELSPFAGR , corresponding to a region within internal sequence amino acids 108 - 157 of Human Nanos Homologue 1 (NANOS1) (NP_955631)

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
  • 组织特异性
    Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
  • 序列相似性
    Belongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • 发展阶段
    Fetal ovary and fetal testis (at protein level).
  • 结构域
    The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
  • 细胞定位
    Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC Rep1a antibody
    • EC_Rep1a antibody
    • NANO1_HUMAN antibody
    • NANOS 1 antibody
    • Nanos homolog 1 (Drosophila) antibody
    • Nanos homolog 1 antibody
    • NANOS1 antibody
    • Nos 1 antibody
    • NOS-1 antibody
    • Nos1 antibody
    • OTTHUMP00000046853 antibody
    • Rep 1a antibody
    • Rep1a antibody
    see all

图片

  • Anti-Nanos Homologue 1 (NANOS1) antibody (ab83417) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Amadei G  et al. A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis. J Neurosci 35:15666-81 (2015). Read more (PubMed: 26609159) »
  • Miles WO  et al. Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells. EMBO J 33:2201-15 (2014). Read more (PubMed: 25100735) »

See all 2 Publications for this product

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