Anti-Nanos Homologue 1 (NANOS1)抗体(ab36163)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-Nanos Homologue 1 (NANOS1)抗体
参阅全部 Nanos Homologue 1 (NANOS1) 一抗 -
描述山羊多克隆抗体to Nanos Homologue 1 (NANOS1)
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宿主Goat
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特异性This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
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经测试应用适用于: ELISA, WB, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide:
SARDGPPGKKLR
, corresponding to amino acids 281-292 of Human Nanos Homologue 1 (NANOS1) -
阳性对照
- Human testes lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab36163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | ||
| WB | ||
| IHC-P |
应用说明
ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
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组织特异性Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
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序列相似性Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
发展阶段Fetal ovary and fetal testis (at protein level).
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结构域The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN. -
细胞定位Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
- Information by UniProt
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数据库链接
- Entrez Gene: 340719 Human
- Omim: 608226 Human
- SwissProt: Q8WY41 Human
- Unigene: 591918 Human
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别名
- EC Rep1a antibody
- EC_Rep1a antibody
- NANO1_HUMAN antibody
see all
图片
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Western blot - Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163)Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) at 0.3 µg/ml (incubated for 1 hour) + Human Testis lysate in RIPA buffer. at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
文献
This product has been referenced in:
- Bonnomet A et al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P ; Human . Read more (PubMed: 18223680) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"