Anti-Nanos Homologue 1 (NANOS1)抗体(ab36163)

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概述

  • 产品名称
    Anti-Nanos Homologue 1 (NANOS1)抗体
    参阅全部 Nanos Homologue 1 (NANOS1) 一抗
  • 描述
    山羊多克隆抗体to Nanos Homologue 1 (NANOS1)
  • 特异性
    This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    SARDGPPGKKLR

    , corresponding to amino acids 281-292 of Human Nanos Homologue 1 (NANOS1)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human testes lysate

性能

应用

Our Abpromise guarantee covers the use of ab36163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
IHC-P
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
    • 组织特异性
      Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
    • 序列相似性
      Belongs to the nanos family.
      Contains 1 nanos-type zinc finger.
    • 发展阶段
      Fetal ovary and fetal testis (at protein level).
    • 结构域
      The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
      The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
    • 细胞定位
      Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
    • Information by UniProt
    • 数据库链接
      • 别名
        • EC Rep1a antibody
        • EC_Rep1a antibody
        • NANO1_HUMAN antibody
        • NANOS 1 antibody
        • Nanos homolog 1 (Drosophila) antibody
        • Nanos homolog 1 antibody
        • NANOS1 antibody
        • Nos 1 antibody
        • NOS-1 antibody
        • Nos1 antibody
        • OTTHUMP00000046853 antibody
        • Rep 1a antibody
        • Rep1a antibody
        see all

      Anti-Nanos Homologue 1 (NANOS1) antibody 图像

      • Western blot - Nanos Homologue 1 (NANOS1) antibody (ab36163)
        Western blot - Nanos Homologue 1 (NANOS1) antibody (ab36163)
        Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) at 0.3 µg/ml (incubated for 1 hour) + Human Testis lysate in RIPA buffer. at 35 µg
        Developed using the ECL technique

        Predicted band size : 30 kDa
        Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163)参考文献

      This product has been referenced in:
      • Bonnomet A  et al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P ; Human . Read more (PubMed: 18223680) »

      See 1 Publication for this product

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