概述

  • 产品名称
    Anti-NANOGP8抗体
  • 描述
    兔多克隆抗体to NANOGP8
  • 特异性
    ab74096 detects endogenous levels of total NANOGP8 protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from an internal region of human NANOGP8.

  • 阳性对照
    • Extracts from Jurkat, HUVEC, HT-29 and HepG2 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.

靶标

  • 相关性
    NANOGP8 may act as a transcription regulator. When overexpressed, promotes cells to enter into S phase and proliferation. Expressed in tumor uterine cervix, breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • NANOG antibody
    • Nanog homeobox pseudogene 8 antibody
    • NANOGP1 antibody
    • NANOGP8 antibody

图片

  • All lanes : Anti-NANOGP8 antibody (ab74096) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells with no peptide
    Lane 2 : Extracts from HUVEC cells with no peptide
    Lane 3 : Extracts from HT-29 cells with no peptide
    Lane 4 : Extracts from HepG2 cells with no peptide
    Lane 5 : Extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Jiang Z  et al. Oncogenic NanogP8 expression regulates cell proliferation and migration through the Akt/mTOR signaling pathway in human gastric cancer - SGC-7901cell line. Onco Targets Ther 9:4859-66 (2016). Read more (PubMed: 27563247) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your patience. The lab told me that they didn't test the antibody to check reactivity with Nanog. Thus, we do not have any data to prove that the antibody will not crossreact with Nanog.   Due to the high homology between both proteins it...

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Thank you for contacting us. I was in touch with the originating lab, and received the following information: "This antibody is specific to NANOGP8. The sequence homology is 92% with NANOG." I have asked them again if they have done any tests to excl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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