概述

  • 产品名称
    Anti-Nanog抗体[NNG-811]
    参阅全部 Nanog 一抗
  • 描述
    小鼠单克隆抗体[NNG-811] to Nanog
  • 经测试应用
    适用于: WB, ICC/IF, IHC-P, IP, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human Nanog.

  • 阳性对照
    • Extracts of NT2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab62734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

图片

  • Immunohistochemical analysis of PFA-fixed paraffin-embedded human neuronal tissue sections labelling Nanog with ab62734 at 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with Citric buffer at pH6.0. Blocking was with 10% serum incubated for 1 hour at 20°C. Secondary used was a rabbit anti-mouse HRP conjugate at 1/200.

    See Abreview

  • 3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
  • Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
    Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
    Lane 3 : Negative control – no primary antibody

    Lane 1 : Cell extract of NT2 cells
    Lane 2 : Cell extract of NT2 cells
    Lane 3 : Cell extract of NT2 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Kakiuchi S  et al. NANOG Expression as a Responsive Biomarker during Treatment with Hedgehog Signal Inhibitor in Acute Myeloid Leukemia. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28245563) »
  • Hossini AM  et al. PI3K/AKT Signaling Pathway Is Essential for Survival of Induced Pluripotent Stem Cells. PLoS One 11:e0154770 (2016). ICC . Read more (PubMed: 27138223) »

See all 14 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human glioblastoma xenograft in nude mice)
Specification
human glioblastoma xenograft in nude mice
Fixative
Alcool-Formol-Acetate (AFA)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
Yes - Triton 0.1%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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