Anti-Nanog抗体- ChIP Grade (ab21624)

概述

  • 产品名称Anti-Nanog抗体- ChIP Grade
    参阅全部 Nanog 一抗
  • 描述
    兔多克隆抗体to Nanog - ChIP Grade
  • 经测试应用适用于: IHC-P, ICC, Flow Cyt, ICC/IF, WB, Sandwich ELISA, ChIPmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Nanog aa 29-49.
    Sequence:

    CGPEENYPSLQMSSAEMPHTE

性能

应用

Our Abpromise guarantee covers the use of ab21624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC 1/100.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/1000.
WB 1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).

Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk.

Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.
ChIP Use at an assay dependent concentration. PubMed: 18710938

靶标

  • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody - ChIP Grade 图像

  • All lanes : Anti-Nanog antibody - ChIP Grade (ab21624) at 1/200 dilution

    Lane 1 : Human fetal fibroblast lysate
    Lane 2 : Human iPS cell lysate


    Predicted band size : 34 kDa
  • All lanes : Anti-Nanog antibody - ChIP Grade (ab21624) at 1/1000 dilution

    Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
    Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.


    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot.
  • ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
  • Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.

  • ab21624 staining Nanog in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.

实验方案

Anti-Nanog antibody - ChIP Grade (ab21624)参考文献

This product has been referenced in:
  • Caiazzo M  et al. Defined three-dimensional microenvironments boost induction of pluripotency. Nat Mater N/A:N/A (2016). IF ; Mouse . Read more (PubMed: 26752655) »
  • Zhang L  et al. CXCR4 activation promotes differentiation of human embryonic stem cells to neural stem cells. Neuroscience N/A:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27615032) »

See all 38 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (iPSCs)
Permeabilization Yes - 0.2% Triton for 10 min
Specification iPSCs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Mr. Mick Wauchope

Verified customer

提交于 Mar 16 2016

Application Western blot
Sample Human Tissue lysate - whole (human liver cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification human liver cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 26 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (iCell® iPSC Diff Day 0)
Permeabilization No
Specification iCell® iPSC Diff Day 0
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Aug 11 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (Human embryonic stem cells)
Specification Human embryonic stem cells
Permeabilization Yes - Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 15 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (liver)
Specification liver
Fixative Paraformaldehyde
Permeabilization Yes - triton-100
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mouse ES cells exogenously expressing Human Nanog)
Specification Mouse ES cells exogenously expressing Human Nanog
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton-X
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Oct 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (liver cancer)
Specification liver cancer
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Detection step Other
Positive control Input
Negative control IgG
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (liver cancer)
Specification liver cancer
Fixative Paraformaldehyde
Permeabilization Yes - Triton-100
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (liver cancer)
Loading amount 10 µg
Specification liver cancer
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 11 2012

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34222 and one vial of ab13537.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"