Anti-Nanog抗体(Biotin) (ab84231)

概述

  • 产品名称Anti-Nanog抗体(Biotin)
    参阅全部 Nanog 一抗
  • 描述
    兔多克隆抗体to Nanog (Biotin)
  • 偶联物Biotin
  • 特异性ab84231 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Nanog in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
  • 经测试应用适用于: WB, ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant human Nanog

  • 阳性对照
    • Recombinant human Nanog.

性能

应用

Our Abpromise guarantee covers the use of ab84231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Sandwich ELISA
  • 应用说明ELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 35 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • 序列相似性Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    Anti-Nanog antibody (Biotin) (ab84231)参考文献

    ab84231 has not yet been referenced specifically in any publications.

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