概述

  • 产品名称Anti-Nanog抗体
    参阅全部 Nanog 一抗
  • 描述
    小鼠单克隆抗体to Nanog
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: TRTVFSSTQL CVLNDRFQRQ KYLSLQQMQE LSNILNLSYK QVKTWFQNQR MKSKRWQKNN WPKNSNGVTQ KASAPTYPSL YSSYHQGCLV NPTGNLPM, corresponding to amino acids 98-195 of Human Nanog (NP_079141) with a proprietary tag.

  • 阳性对照
    • HeLa nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab89500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Nanog is approximately 0.3ng/ml when used as a capture antibody.

靶标

  • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody 图像

Anti-Nanog antibody (ab89500)参考文献

This product has been referenced in:
  • Huang ZJ  et al. Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells. Mol Med Rep 11:1647-54 (2015). WB ; Human . Read more (PubMed: 25405855) »

See 1 Publication for this product

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