概述

  • 产品名称Anti-Nanog抗体
    参阅全部 Nanog 一抗
  • 描述
    兔多克隆抗体to Nanog
  • 经测试应用适用于: Flow Cyt, IHC - Wholemount, ICC, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Mouse Nanog protein

性能

应用

Our Abpromise guarantee covers the use of ab80892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
ICC 1/150 - 1/700.
WB 1/300 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody 图像

  • ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Tween 20 prior to blocking in 20% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25°C. A Cy5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary. Nuclear staining with DAPI.

    See Abreview

  • IHC-Wholemount image of anti-Nanog antibody (ab80892) staining on a mouse pre-implantation embryo. This embryo was fixed in paraformaldehyde, permeabilized in 0.25% Triton X and then blocked in 5% serum for 1 hour at room temperature. It was stained with anti-Nanog antibody overnight at 4 degrees (right), and DAPI (left).

    See Abreview

  • ab80892 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab80892 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Nanog antibody (ab80892) at 1/500 dilution

    Lane 1 : Marker
    Lane 2 : Mouse embryonic stem cell line D3.
    Lane 3 : Mouse embryonic stem cell line D3.
    Lane 4 : Mutant mouse embryonic stem cell line known to not express nanog.
    Lane 5 : Mutant mouse embryonic stem cell line known to not express nanog.

    Lysates/proteins at 200000 cells per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous abreview.

    See Abreview

  • Overlay histogram of Nanog staining in mouse embryonic stem cells using ab80892, at 1:100 dilution. Purple histogram represents negative control (rabbit IgG); green line represents anti-Nanog antibody (ab80892).

    See Abreview

Anti-Nanog antibody (ab80892)参考文献

This product has been referenced in:
  • Caiazzo M  et al. Defined three-dimensional microenvironments boost induction of pluripotency. Nat Mater N/A:N/A (2016). IF ; Mouse . Read more (PubMed: 26752655) »
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »

See all 52 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Rabbit Cultured Cells (brain)
Permeabilization Yes - tritonx100
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

提交于 Oct 31 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative 10% Formalin
Username

Abcam user community

Verified customer

提交于 Apr 25 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic)
Permeabilization Yes - Triton-X100 (0.5%)
Specification Embryonic
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 23 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (mouse cerebellum)
Specification mouse cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 18 2014

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.5% v/v tween20 in PBS
Sample Mouse Cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Gating Strategy live cells
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.5% v/v tween20 in PBS
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Permeabilization Yes - with Tris and Tween
Blocking step Peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (embryonic testis E92)
Specification embryonic testis E92
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

提交于 Feb 11 2013

DISCOUNT CODE: ABTRIAL-EBAPI
Ablaufdatum: 27/11/12

Die Frau Hoffmann ist zurzeit leider außer Haus, daher habe ich den AbTrial Code für Sie ausgestellt.

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Ich freue mich Ihnen mitteilen zu können, dass ab80892 (Anti-Nanog antibody) der selbe Antikörper ist wie ab21603. Bei ab80892 handelt um eine neue Ch...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"