概述

  • 产品名称Anti-Nanog抗体
    参阅全部 Nanog 一抗
  • 描述
    兔多克隆抗体to Nanog
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog.

    .

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Mouse Embryonic Stem Cell.

性能

应用

Our Abpromise guarantee covers the use of ab14959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • 序列相似性Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    Anti-Nanog antibody 图像

    • All lanes : Anti-Nanog antibody (ab14959) at 1 µg/ml

      Lane 1 : As above
      Lane 2 : As above
      Lane 3 : Mouse Nanog peptide (ab14960) at 1 µg/ml
      Lane 4 : Mouse Nanog peptide (ab14960) at 1 µg/ml


      Predicted band size : 34 kDa
      Observed band size : 34.6 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 18 kDa (possible cross reactivity),38 kDa (possible cross reactivity),50 kDa (possible cross reactivity).

      Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
      Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml

      Observed band size: 34.2 kDa
      Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).

      Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
      Lanes 2 and 4: Human embryonic stem cell lysate (negative control)

      ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.

      Performed under reducing conditions.

    • All lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml

      Lane 1 : ES Cell Nuclear Extract
      Lane 2 : ES Cell Nuclear Extract


      Predicted band size : 34 kDa

      This image is courtesy of Adam Yates and Ian Chambers, University of Edinburgh

    • ab14959 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab14959 at 1/600 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

      See Abreview

    Anti-Nanog antibody (ab14959)参考文献

    This product has been referenced in:
    • Ye S  et al. Pleiotropy of Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Self-Renewal of Embryonic Stem Cells from Refractory Mouse Strains. PLoS One 7:e35892 (2012). ICC/IF . Read more (PubMed: 22540008) »
    • Osorno R  et al. The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression. Development 139:2288-98 (2012). Read more (PubMed: 22669820) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your response.

    This antibody has been raised using a synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog. Your question seems to be about its specificity (species-specificity) and not its sensitivity. Read More

    I am sorry to hear that you have been experiencing problems using this product in the application/species that you wish.

    Could you provide some further details of the protocol used and complete the following form (attached as a word document)...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Teratoma)
    Specification Teratoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Apr 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (ES cell)
    Loading amount 10 µg
    Specification ES cell
    Treatment 2000 U/ml mouse LIF constantly
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 12 2009

    Thank you for your enquiry. There are at least three isotype controls you could use: normal rabbit serum (ab7487), ab27472, ab27478. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Thank you for your enquiry and your patience in waiting for my reply. I have compared the immunogen sequences with the sequence of bovine nanog using a ClustalW alignment. For ab14959 less than 30% of the sequence is homologous, for ab21624 the hom...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"