概述

  • 产品名称Anti-Nanog抗体
    参阅全部 Nanog 一抗
  • 描述
    兔多克隆抗体to Nanog
  • 特异性At least two isoforms of Nanog are known to exist; ab106465 will detect both.
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 19 amino acid synthetic peptide near the center of Human Nanog (EAW88651).

  • 阳性对照
    • Human spleen tissue lysate; Human spleen tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab106465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.

靶标

  • 功能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 组织特异性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 序列相似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 发展阶段Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody 图像

  • Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/ml
    Lane 2 : Anti-Nanog antibody (ab106465) at 2 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 35 kDa
  • ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.
  • Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.

Anti-Nanog antibody (ab106465)参考文献

This product has been referenced in:
  • Anchan RM  et al. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS One 9:e115106 (2014). ICC/IF ; Mouse . Read more (PubMed: 25517354) »

See 1 Publication for this product

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I am sorry to hear that you have been experiencing problems with this antibody. The antibody was delivered on Friday and was received by you on Monday. Now not storing the products as recommended on the datasheet actually voids the Abpromise guarantee ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"