概述

  • 产品名称Anti-NALP12抗体
    参阅全部 NALP12 一抗
  • 描述
    兔多克隆抗体to NALP12
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog, Pig, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NALP12.

  • 阳性对照
    • This antibody gave a positive signal in both HL60 and Jurkat whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab100863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK.
  • 组织特异性Detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes.
  • 疾病相关Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2) [MIM:611762]. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
  • 序列相似性Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLR19.3 antibody
    • FCAS2 antibody
    • Monarch 1 antibody
    • Monarch-1 antibody
    • NACHT, leucine rich repeat and PYD containing 12 antibody
    • NACHT, LRR and PYD containing protein 12 antibody
    • NACHT, LRR and PYD domains-containing protein 12 antibody
    • NAL12_HUMAN antibody
    • NLR family, pyrin domain containing 12 antibody
    • NLRP12 antibody
    • Nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12 antibody
    • OTTHUMP00000067227 antibody
    • PAN6 antibody
    • PYPAF7 antibody
    • PYRIN containing APAF1 like protein 7 antibody
    • PYRIN-containing APAF1-like protein 7 antibody
    • Regulated by nitric oxide antibody
    • RNO antibody
    • RNO2 antibody
    see all

Anti-NALP12 antibody 图像

  • All lanes : Anti-NALP12 antibody (ab100863) at 1 µg/ml (Milk Blocking - 3%)

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 68 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of NALP12 staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab100863, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NALP12 antibody (ab100863)参考文献

ab100863 has not yet been referenced specifically in any publications.

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