概述

  • 产品名称Anti-NAIP抗体
    参阅全部 NAIP 一抗
  • 描述
    兔多克隆抗体to NAIP
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAIP.

  • 阳性对照
    • This antibody gave a positive signal in Human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab98020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).

靶标

  • 功能Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
  • 组织特异性Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
  • 序列相似性Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Baculoviral IAP repeat containing 1 antibody
    • Baculoviral IAP repeat-containing protein 1 antibody
    • BIRC 1 antibody
    • BIRC1 antibody
    • BIRC1_HUMAN antibody
    • Birc1a antibody
    • FLJ42520 antibody
    • NAIP antibody
    • Naip1 antibody
    • Neuronal apoptosis inhibitory protein antibody
    • NLR family apoptosis inhibitory protein antibody
    • NLR family BIR domain containing 1 antibody
    • NLRB 1 antibody
    • NLRB1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
    • Psi neuronal apoptosis inhibitory protein antibody
    • psiNAIP antibody
    • Similar to occludin antibody
    see all

Anti-NAIP antibody 图像

  • Anti-NAIP antibody (ab98020) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 160 kDa
    Observed band size : 160 kDa
    Additional bands at : 31 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab98020 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98020, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.

Anti-NAIP antibody (ab98020)参考文献

ab98020 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab98020.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"