概述

  • 产品名称Anti-NAIP抗体
    参阅全部 NAIP 一抗
  • 描述
    兔多克隆抗体to NAIP
  • 特异性ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).

  • 阳性对照
    • PC-3 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab25968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).Can be blocked with Human NAIP peptide (ab39795).
IHC-P Use at an assay dependent concentration. PubMed: 20844241
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
  • 组织特异性Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
  • 序列相似性Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Baculoviral IAP repeat containing 1 antibody
    • Baculoviral IAP repeat-containing protein 1 antibody
    • BIRC 1 antibody
    • BIRC1 antibody
    • BIRC1_HUMAN antibody
    • Birc1a antibody
    • FLJ42520 antibody
    • NAIP antibody
    • Naip1 antibody
    • Neuronal apoptosis inhibitory protein antibody
    • NLR family apoptosis inhibitory protein antibody
    • NLR family BIR domain containing 1 antibody
    • NLRB 1 antibody
    • NLRB1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
    • Psi neuronal apoptosis inhibitory protein antibody
    • psiNAIP antibody
    • Similar to occludin antibody
    see all

Anti-NAIP antibody 图像



  • Predicted band size : 160 kDa
    Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
    Minor, lower molecular weight bands may represent alternately spliced forms.
  • ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF

  • IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 160 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa

    See Abreview

  • Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.

Anti-NAIP antibody (ab25968)参考文献

This product has been referenced in:
  • Zhang S  et al. Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma. Oncotarget 5:7183-97 (2014). Read more (PubMed: 25216531) »
  • Petterson T  et al. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J Leukoc Biol 89:177-87 (2011). IHC-P ; Human . Read more (PubMed: 20844241) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - other (RAW 264.7)
Loading amount 20 µg
Specification RAW 264.7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Apr 27 2012

Thank you for your enquiry. I have enquired at the originator of the product for the WB protocols. Please see below for further details. I hope this information will be helpful. To answer your questions: 1. We us an anti-rabbit IgG, HRP con...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"