Anti-NAIP抗体(ab25968)

Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1µg/ml.

Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms.

IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg

Secondary
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 160 kDa
Observed band size: 150 kDa (why is the actual band size different from the predicted?)
Additional bands at: 110 kDa (possible non-specific binding)


Exposure time: 1 minute

See Abreview

Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.