Customer reviews and FAQs

概述

  • 产品名称
    Anti-NAIP抗体
    参阅全部 NAIP 一抗
  • 描述
    兔多克隆抗体to NAIP
  • 特异性
    ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).
  • 经测试应用
    适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).

  • 阳性对照
    • PC-3 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab25968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).Can be blocked with Human NAIP peptide (ab39795).
IHC-P Use at an assay dependent concentration. PubMed: 20844241
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
  • 组织特异性
    Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
  • 序列相似性
    Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Baculoviral IAP repeat containing 1 antibody
      • Baculoviral IAP repeat-containing protein 1 antibody
      • BIRC 1 antibody
      • BIRC1 antibody
      • BIRC1_HUMAN antibody
      • Birc1a antibody
      • FLJ42520 antibody
      • NAIP antibody
      • Naip1 antibody
      • Neuronal apoptosis inhibitory protein antibody
      • NLR family apoptosis inhibitory protein antibody
      • NLR family BIR domain containing 1 antibody
      • NLRB 1 antibody
      • NLRB1 antibody
      • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
      • Psi neuronal apoptosis inhibitory protein antibody
      • psiNAIP antibody
      • Similar to occludin antibody
      see all

    Anti-NAIP antibody 图像

    • Western blot - Anti-NAIP antibody (ab25968)
      Western blot - Anti-NAIP antibody (ab25968)


      Predicted band size : 160 kDa
      Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1µg/ml.
    • Western blot - Anti-NAIP antibody (ab25968)
      Western blot - Anti-NAIP antibody (ab25968)


      Predicted band size : 160 kDa
      Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
      Minor, lower molecular weight bands may represent alternately spliced forms.
    • Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (ab25968)
      Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (ab25968)

      ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAIP antibody (ab25968)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAIP antibody (ab25968)
      IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-NAIP antibody (ab25968)
      Western blot - Anti-NAIP antibody (ab25968)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa
      Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg

      Secondary
      HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 160 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa (possible non-specific binding).

      Exposure time : 1 minute

      This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappa

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (ab25968)
      Immunocytochemistry/ Immunofluorescence - Anti-NAIP antibody (ab25968)
      Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.

    Anti-NAIP antibody (ab25968)参考文献

    This product has been referenced in:
    • Zhang S  et al. Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma. Oncotarget 5:7183-97 (2014). Read more (PubMed: 25216531) »
    • Petterson T  et al. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J Leukoc Biol 89:177-87 (2011). IHC-P ; Human . Read more (PubMed: 20844241) »

    See all 5 Publications for this product

    发表研究结果有使用 ab25968？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    2 Abreviews or Q&A

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    Western blot abreview for Anti-NAIP antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (RAW 264.7)
    Loading amount
    20 µg
    Specification
    RAW 264.7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    提交于 Apr 27 2012

    First of all, I find it exceedingly difficult to ask a simple question regarding products that have been ordered from your compnay and paid for already (one of these not yet been received). Anyway, my question is rather simple, I have purchased the ant...

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    Thank you for your enquiry. I have enquired at the originator of the product for the WB protocols. Please see below for further details. I hope this information will be helpful. To answer your questions: 1. We us an anti-rabbit IgG, HRP con...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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