概述

  • 产品名称
  • 描述
    兔多克隆抗体to NAF1
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: EIFGPVAHPF YVLRFNSSDH IESKGIKIKE TMYFAPSMKD FTQYIFTEKL KQDKGSDASW KNDQEPPPEA LDFSDDEKEK EAKQRKKSQI QGRK, corresponding to amino acids 242-335 of Human NAF1 (Q96HR8).

  • 阳性对照
    • Human esophagus tissue and human cell line U-2 OS

性能

应用

Our Abpromise guarantee covers the use of ab122323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

  • 功能
    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
  • 序列相似性
    Belongs to the NAF1 family.
  • 细胞定位
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
    • hNAF1 antibody
    • Naf1 antibody
    • NAF1_HUMAN antibody
    • Nuclear assembly factor 1 homolog antibody
    • Nuclear assembly factor 1 ribonucleoprotein antibody
    see all

Anti-NAF1 antibody 图像

  • Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells
  • Immunofluorescent staining of human cell line U-2 OS (Fixation/Permeabilization: PFA/Triton X-100) with ab122323 at 4ug/ml shows positivity in cytoplasm, centrosome & cell junctions. Antibody staining is shown in green.

Anti-NAF1 antibody (ab122323)参考文献

ab122323 has not yet been referenced specifically in any publications.

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