Anti-NADH2抗体(ab102753)

概述

  • 产品名称Anti-NADH2抗体
  • 描述
    兔多克隆抗体to NADH2
  • 经测试应用适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 38-67 of Human NADH2 (P03891)

  • 阳性对照
    • K562 cell lysate and human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab102753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性NADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
  • 细胞定位Mitochondrion inner membrane; Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • MTND2 antibody
    • NADH dehydrogenase subunit 2 antibody
    • NADH ubiquinone oxidoreductase chain 2 antibody
    • ND2 antibody

Anti-NADH2 antibody 图像

  • Anti-NADH2 antibody (ab102753) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 39 kDa
  • Formalin-fixed and paraffin-embedded human brain tissue labelled with ab102753 at 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using ab102753 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-NADH2 antibody (ab102753)参考文献

ab102753 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"