NADA (N-Arachidonyldopamine),endogenous CB1 / TRPV1激动剂(ab120099)
Key features and details
- Endogenous CB1 / TRPV1 agonist
- CAS Number: 199875-69-9
- Supplied in ethanol (5 mg/ml)
- Form / State: Liquid
- Source: Synthetic
概述
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产品名称
NADA (N-Arachidonyldopamine),endogenous CB1 / TRPV1激动剂 -
描述
Endogenous CB1 / TRPV1激动剂 -
别名
- N-Arachidonyldopamine
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CAS编号
199875-69-9 -
化学结构
性能
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化学名称
(5Z,8Z,11Z,14Z)-N-(3,5-dihydroxyphenethyl)icosa-5,8,11,14-tetraenamide -
分子量
439.63 -
分子式
C28H41NO3 -
PubChem识别号
5282105 -
存放说明
Store at -20°C (desiccating conditions). -
溶解度概述
Supplied in ethanol (5 mg/ml) -
处理
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc1ccc(CCNC(=O)CCC/C=CC/C=CC/C=CC/C=CCCCCC)cc1O -
来源
Synthetic
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研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Vitamin D Receptor
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120099, NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist
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ab15580 staining Ki67 in SK-N-SH cells treated with NADA (N-Arachidonyldopamine) (ab120099), by ICC/IF. Decrease in Ki67 expression correlates with increased concentration of NADA (N-Arachidonyldopamine), as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120099 (NADA (N-Arachidonyldopamine)) in ethanol, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15580 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120099 被引用在 1 文献中.
- Ferreira SG et al. N-acyldopamines control striatal input terminals via novel ligand-gated cation channels. Neuropharmacology 56:676-83 (2009). PubMed: 19101577