Anti-NACA1抗体(ab67872)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to NACA1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length NACA1 protein (Human)

  • 阳性对照
    • NACA1 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能
    Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the NAC-alpha family.
    Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    Contains 1 UBA domain.
  • 翻译后修饰
    Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Also found in nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha NAC antibody
    • Alpha-NAC antibody
    • Hom s 2.02 antibody
    • HSD48 antibody
    • MGC117224 antibody
    • NAC-alpha antibody
    • naca antibody
    • NACA/1.9.2 antibody
    • NACA_HUMAN antibody
    • NACA1 antibody
    • Nascent polypeptide associated complex alpha polypeptide antibody
    • Nascent polypeptide associated complex alpha subunit antibody
    • Nascent polypeptide associated complex subunit alpha antibody
    • Nascent polypeptide-associated complex subunit alpha antibody
    see all

Anti-NACA1 antibody 图像

  • All lanes : Anti-NACA1 antibody (ab67872) at 1/500 dilution

    Lane 1 : NACA1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-NACA1 antibody (ab67872)参考文献

ab67872 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novacastra, pH 6.0
Permeabilization
No
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

提交于 Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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