概述

  • 产品名称
  • 描述
    兔多克隆抗体to Nac1
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Nac1.

    (Peptide available as ab30604.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: F9 (Mouse embryonic carcinoma cell line) Whole Cell IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Brain (Rat) Tissue Lysate - normal tissue It also gave a positive result in FFPE human cerebral cortex sections.

性能

应用

Our Abpromise guarantee covers the use of ab29047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 57,60 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
  • 组织特异性
    Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
  • 序列相似性
    Contains 1 BEN domain.
    Contains 1 BTB (POZ) domain.
  • 细胞定位
    Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BEND8 antibody
    • BTB Domain Containing 14B antibody
    • BTB/POZ domain-containing protein 14B antibody
    • btbd14b antibody
    • FLJ37383 antibody
    • NAC-1 antibody
    • NAC1 antibody
    • Nacc1 antibody
    • NACC1_HUMAN antibody
    • Nucleus accumbens-associated protein 1 antibody
    see all

Anti-Nac1 antibody 图像

  • ab29047 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab29047 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Nac1 antibody (ab29047) at 1/250 dilution + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ICC/IF image of ab29047 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29047, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • IHC image of Nac1 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29047, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Nac1 antibody (ab29047)参考文献

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma)
Loading amount
30 µg
Specification
Melanoma
Gel Running Conditions
Reduced Denaturing
Blocking step
casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Yuichi Ishikawa

Verified customer

提交于 Nov 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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