参阅全部 Nab2 一抗
WB, ELISAmore details
Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee
A region within synthetic peptide: MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ, corresponding to N terminal amino acids 1-50 of Human Nab2
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Protein A purified
- Compatible Secondaries
- Positive Controls
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
- 功能Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
- 组织特异性Widely expressed at low levels. Highly expressed in melanoma cell lines.
- 序列相似性Belongs to the NAB family.
- 结构域The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
- 细胞定位Nucleus. Isoform 2 is not localized to the nucleus.
Information by UniProt
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
Anti-Nab2 antibody 图像
Western blot - Nab2 antibody (ab66604)
All lanes : Anti-Nab2 antibody (ab66604) at 1/1.25 dilution (in 5% skim milk / PBS buffer)
Lane 1 : marker
Lane 2 : HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 48,62 kDa (why is the actual band size different from the predicted?)
Anti-Nab2 antibody (ab66604)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"