概述

  • 产品名称Anti-Nab2抗体
    参阅全部 Nab2 一抗
  • 描述
    兔多克隆抗体to Nab2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ, corresponding to N terminal amino acids 1-50 of Human Nab2

  • 阳性对照
    • HepG2 cells

性能

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应用

Our Abpromise guarantee covers the use of ab66604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

靶标

  • 功能Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • 组织特异性Widely expressed at low levels. Highly expressed in melanoma cell lines.
  • 序列相似性Belongs to the NAB family.
  • 结构域The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • 细胞定位Nucleus. Isoform 2 is not localized to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

Anti-Nab2 antibody 图像

  • All lanes : Anti-Nab2 antibody (ab66604) at 1/1.25 dilution (in 5% skim milk / PBS buffer)

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 48,62 kDa (why is the actual band size different from the predicted?)

Anti-Nab2 antibody (ab66604)参考文献

ab66604 has not yet been referenced specifically in any publications.

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