Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee
A region within synthetic peptide: MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ, corresponding to N terminal amino acids 1-50 of Human Nab2
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Widely expressed at low levels. Highly expressed in melanoma cell lines.
Belongs to the NAB family.
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Nucleus. Isoform 2 is not localized to the nucleus.
Information by UniProt
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
Anti-Nab2 antibody 图像
Western blot - Nab2 antibody (ab66604)
All lanes :
Anti-Nab2 antibody (ab66604) at 1/1.25 dilution (in 5% skim milk / PBS buffer)Lane 1 :
markerLane 2 :
HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
56 kDaObserved band size :
48,62 kDa (why is the actual band size different from the predicted?
Anti-Nab2 antibody (ab66604)参考文献
has not yet been referenced specifically in any publications.
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