Anti-Nab2抗体(ab112056)
Key features and details
- Rabbit polyclonal to Nab2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Nab2抗体 -
描述
兔多克隆抗体to Nab2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 475-525 of Human Nab2 (NP_005958.1)
-
阳性对照
- Whole cell lysate from 293T and Jurkat cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112056于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
功能
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability. -
组织特异性
Widely expressed at low levels. Highly expressed in melanoma cell lines. -
序列相似性
Belongs to the NAB family. -
结构域
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression. -
细胞定位
Nucleus. Isoform 2 is not localized to the nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4665 Human
- Entrez Gene: 17937 Mouse
- Entrez Gene: 314910 Rat
- Entrez Gene: 714209 Rhesus monkey
- Omim: 602381 Human
- SwissProt: Q15742 Human
- SwissProt: Q61127 Mouse
- Unigene: 159223 Human
see all -
别名
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
see all
图片
-
All lanes : Anti-Nab2 antibody (ab112056) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Predicted band size: 57 kDa
Exposure time: 3 minutes -
Immunoprecipitation of NAB2. Samples: 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 µg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 µg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab112056 尚未被引用在任何文献中。