概述

  • 产品名称Anti-N4BP1抗体
    参阅全部 N4BP1 一抗
  • 描述
    兔多克隆抗体to N4BP1
  • 特异性This antibody detects endogenous levels of total N4BP1 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthesized peptide derived from an internal region of human N4BP1.

  • 阳性对照
    • Extracts from MCF-7 cells, 293 cells and Jurkat cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24831002
WB 1/500 - 1/1000. Predicted molecular weight: 100 kDa.
ELISA 1/40000.

靶标

  • 功能Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • 组织特异性Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • 序列相似性Belongs to the N4BP1 family.
  • 翻译后修饰Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody 图像

  • All lanes : Anti-N4BP1 antibody (ab74260) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from Jurkat cells
    Lane 4 : Extracts from MCF-7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 100 kDa
    Observed band size : 100 kDa

Anti-N4BP1 antibody (ab74260)参考文献

This product has been referenced in:
  • Sugeno N  et al. Lys-63-linked Ubiquitination by E3 Ubiquitin Ligase Nedd4-1 Facilitates Endosomal Sequestration of Internalized a-Synuclein. J Biol Chem 289:18137-18151 (2014). WB, ICC/IF . Read more (PubMed: 24831002) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed aga...

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Thank you for contacting Abcam regarding ab74260. I am sorry that you are experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with...

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