概述

  • 产品名称Anti-N4BP1抗体
    参阅全部 N4BP1 一抗
  • 描述
    兔多克隆抗体to N4BP1
  • 特异性At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform.
  • 经测试应用适用于: ICC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab106649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.

靶标

  • 功能Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
  • 组织特异性Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
  • 序列相似性Belongs to the N4BP1 family.
  • 翻译后修饰Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ31821 antibody
    • KIAA0615 antibody
    • MGC176730 antibody
    • N4BP1 antibody
    • N4BP1_HUMAN antibody
    • NEDD4 binding protein 1 antibody
    • NEDD4-binding protein 1 antibody
    see all

Anti-N4BP1 antibody 图像

  • Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.
  • All lanes : Anti-N4BP1 antibody (ab106649) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 100 kDa
  • Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.

Anti-N4BP1 antibody (ab106649)参考文献

ab106649 has not yet been referenced specifically in any publications.

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