Anti-N WASP (phospho Y256)抗体(ab23395)

概述

  • 产品名称Anti-N WASP (phospho Y256)抗体
    参阅全部 N WASP 一抗
  • 描述
    兔多克隆抗体to N WASP (phospho Y256)
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Phospho N WASP (Tyr 256) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around tyrosine 256 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding tyrosine 291.

  • 阳性对照
    • Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab23395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.
ELISA 1/20000.

靶标

  • 功能Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
  • 序列相似性Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 2 WH2 domains.
  • 细胞定位Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp779G0847 antibody
    • MGC48327 antibody
    • N-WASP antibody
    • Neural Wiskott Aldrich syndrome protein antibody
    • Neural Wiskott-Aldrich syndrome protein antibody
    • NWASP antibody
    • Wasl antibody
    • WASL_HUMAN antibody
    • WASPB antibody
    • Wiskott Aldrich syndrome gene like antibody
    • Wiskott Aldrich syndrome gene like protein antibody
    • Wiskott Aldrich syndrome like antibody
    • WiskottAldrich syndrome like antibody
    see all

Anti-N WASP (phospho Y256) antibody 图像

  • All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution

    Lane 1 : Control A431 cells 20ug/lane
    Lane 2 : Pervanadate-treated A431 cells 20 ug/lane


    Predicted band size : 55 kDa

Anti-N WASP (phospho Y256) antibody (ab23395)参考文献

This product has been referenced in:
  • Zhang W  et al. p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization. J Physiol 594:4879-900 (2016). Read more (PubMed: 27038336) »
  • Rajput C  et al. Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. J Biol Chem 288:4241-50 (2013). WB ; Human . Read more (PubMed: 23212915) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. ab23395 was raised using a phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"