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Our Abpromise guarantee covers the use of ab56454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||Use at an assay dependent concentration. PubMed: 18641286|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: N WASP knockout HAP1 cell lysate (20 µg)
Lane 3: A549 whole cell lysate (20 µg)
Lane 4: Human thyroid gland (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab56454 observed at 66 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab56454 was shown to specifically react with N WASP when N WASP knockout samples were used. Wild-type and N WASP knockout samples were subjected to SDS-PAGE. ab56454 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"