概述

  • 产品名称Anti-N WASP抗体
    参阅全部 N WASP 一抗
  • 描述
    小鼠单克隆抗体to N WASP
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Recombinant fragment: NFVYNSPRGY FHTFAGDTCQ VALNFANEEE AKKFRKAVTD LLGRRQRKSE KRRDPPNGPN LPMATVDIKN PEITTNRFYG PQVNNISH, corresponding to amino acids 97-185 of Human N WASP

性能

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应用

Our Abpromise guarantee covers the use of ab56454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 18641286

靶标

  • 功能Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
  • 序列相似性Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 2 WH2 domains.
  • 细胞定位Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp779G0847 antibody
    • MGC48327 antibody
    • N-WASP antibody
    • Neural Wiskott Aldrich syndrome protein antibody
    • Neural Wiskott-Aldrich syndrome protein antibody
    • NWASP antibody
    • Wasl antibody
    • WASL_HUMAN antibody
    • WASPB antibody
    • Wiskott Aldrich syndrome gene like antibody
    • Wiskott Aldrich syndrome gene like protein antibody
    • Wiskott Aldrich syndrome like antibody
    • WiskottAldrich syndrome like antibody
    see all

Anti-N WASP antibody 图像



  • Predicted band size : 55 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: N WASP knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 whole cell lysate (20 µg)
    Lane 4: Human thyroid gland (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab56454 observed at 66 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab56454 was shown to specifically react with N WASP when N WASP knockout samples were used. Wild-type and N WASP knockout samples were subjected to SDS-PAGE. ab56454 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.



  • Predicted band size : 55 kDa
    N WASP antibody (ab56454) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.

Anti-N WASP antibody (ab56454)参考文献

This product has been referenced in:
  • Aga M  et al. Specialized podosome- or invadopodia-like structures (PILS) for focal trabecular meshwork extracellular matrix turnover. Invest Ophthalmol Vis Sci 49:5353-65 (2008). IHC-P ; Pig . Read more (PubMed: 18641286) »

See 1 Publication for this product

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