Anti-N myc interactor抗体(ab70210)

概述

  • 产品名称Anti-N myc interactor抗体
    参阅全部 N myc interactor 一抗
  • 描述
    兔多克隆抗体to N myc interactor
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 250 and 307 of human N myc interactor. (NP_004679.1)

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • N myc (and STAT) interactor antibody
      • N myc and STAT interactor antibody
      • N myc interactor antibody
      • NMI antibody
      • NMYC and STAT interactor antibody
      • NMYC interactor antibody
      see all

    Anti-N myc interactor antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and bladder carcinoma (right) tissues labelling N myc interactor with ab70210 at 1/1000 (0.2 µg/ml). Detection: DAB.
    • All lanes : Anti-N myc interactor antibody (ab70210) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50
      Lane 4 : Whole cell lysate from 293T cells at 50
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50


      Predicted band size : 35 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    Anti-N myc interactor antibody (ab70210)参考文献

    ab70210 has not yet been referenced specifically in any publications.

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