Anti-N myc interactor抗体(ab70209)

概述

  • 产品名称Anti-N myc interactor抗体
    参阅全部 N myc interactor 一抗
  • 描述
    兔多克隆抗体to N myc interactor
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 (MEADKDDTQQ ILKEHSPDE F IKDEQNKG LI DEITKKN IQL KKEIQK LETE) of human N myc interactor

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • N myc (and STAT) interactor antibody
      • N myc and STAT interactor antibody
      • N myc interactor antibody
      • NMI antibody
      • NMYC and STAT interactor antibody
      • NMYC interactor antibody
      see all

    Anti-N myc interactor antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling N myc interactor with ab70209 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Immunoprecipitation of Human N myc interactor. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-NMI antibody (ab70209) used at 3 µg/ml for IP. Arrows indicate N myc interactor on Lane 1. lane 2 represents IgG IP control. Note: ab70210 at 0.04 µg/ml was used for WB detection.

    Anti-N myc interactor antibody (ab70209)参考文献

    ab70209 has not yet been referenced specifically in any publications.

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