Anti-n-Myc/MYCN抗体[NCM II 100] (ab16898)
Key features and details
- Mouse monoclonal [NCM II 100] to n-Myc/MYCN
- Suitable for: IP, IHC-Fr, ICC/IF, WB, ChIP, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
-
产品名称
Anti-n-Myc/MYCN抗体[NCM II 100]
参阅全部 n-Myc/MYCN 一抗 -
描述
小鼠单克隆抗体[NCM II 100] to n-Myc/MYCN -
宿主
Mouse -
特异性
This antibody reacts with N-myc/MYCN encoded proteins and their cleavage products.
-
经测试应用
适用于: IP, IHC-Fr, ICC/IF, WB, ChIP, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Fusion protein corresponding to Human n-Myc/MYCN. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
Database link: P04198 -
阳性对照
- IMR5 Cells
-
常规说明
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.82% Sodium phosphate, 50% Glycerol -
Concentration information loading...
-
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
NCM II 100 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. |
|
IHC-Fr |
Use at an assay dependent concentration.
See references. |
|
ICC/IF |
Use at an assay dependent concentration.
Pubmed ID: 2426708. |
|
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
See references. |
ChIP |
Use at an assay dependent concentration. PubMed: 19495417
Suggested to be used at 2 ug. |
|
Flow Cyt |
Use at an assay dependent concentration.
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
IP
Use at an assay dependent concentration. It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. |
IHC-Fr
Use at an assay dependent concentration. See references. |
ICC/IF
Use at an assay dependent concentration. Pubmed ID: 2426708. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. See references. |
ChIP
Use at an assay dependent concentration. PubMed: 19495417 Suggested to be used at 2 ug. |
Flow Cyt
Use at an assay dependent concentration. It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
May function as a transcription factor. -
疾病相关
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
发展阶段
Expressed during fetal development. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Omim: 164840 Human
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- Unigene: 25960 Human
- Unigene: 16469 Mouse
-
别名
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
图片
-
Immunoprecipitation of N-Myc/MYCN from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
ab16898 used at 1 mg/sample.This image was generated using the ascites version of the product.
-
Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (39)
ab16898 被引用在 39 文献中.
- Yi JS et al. The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53. Neoplasia 23:624-633 (2021). PubMed: 34107377
- Santiago T et al. Multinational Retrospective Central Pathology Review of Neuroblastoma: Lessons Learned to Establish a Regional Pathology Referral Center in Resource-Limited Settings. Arch Pathol Lab Med 145:214-221 (2021). PubMed: 33501494
- Kilinc S et al. Oncogene-regulated release of extracellular vesicles. Dev Cell 56:1989-2006.e6 (2021). PubMed: 34118203
- Lundsten S et al. p53-Mediated Radiosensitization of 177Lu-DOTATATE in Neuroblastoma Tumor Spheroids. Biomolecules 11:N/A (2021). PubMed: 34827693
- Bian X et al. The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer. Front Oncol 10:565820 (2020). PubMed: 33134168