Anti-N-epsilon acetyl Lysine抗体[11A1] (ab409)

概述

  • 产品名称Anti-N-epsilon acetyl Lysine抗体[11A1]
  • 描述
    小鼠单克隆抗体[11A1] to N-epsilon acetyl Lysine
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Species independent
  • 常规说明The ability of this antibody to fix complement is unknown.

性能

应用

Our Abpromise guarantee covers the use of ab409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000.
ELISA 1/5000 - 1/50000.
IHC-P 1/500 - 1/5000.

Anti-N-epsilon acetyl Lysine antibody [11A1] 图像

  • Image from Pehar M et al., J Biol Chem. 2012 Jun 29;287(27):22436-40. Epub 2012 May 24.; Fig 2.; doi: 10.1074/jbc.C112.362871; June 29, 2012, The Journal of Biological Chemistry, 287, 22436-22440.

Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)参考文献

This product has been referenced in:
  • Peng Y  et al. Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer. J Neurosci 34:6772-89 (2014). Read more (PubMed: 24828632) »
  • Ding Y  et al. Biochemical Inhibition of the Acetyltransferases ATase1 and ATase2 Reduces ß-Secretase (BACE1) Levels and Aß Generation. J Biol Chem 287:8424-33 (2012). WB . Read more (PubMed: 22267734) »

See all 8 Publications for this product

Product Wall

The carboxybenzyloxy (CBZ) is a blocking group used to protect the primary amine during organic synthesis. We have used this derivative for the immunogen synthesis. There was no acetylated group on the immunogen.

The immunogen used to prepare this mouse monoclonal antibody was the KLH-lysine Nalpha-CBZ If you have any additional questions, please contact us again.

Ab409 used to be sold in lyophilized form and is now sold as a liquid. I apologize for the mistake on the datasheet and it has since been updated. Would you like a replacement vial of ab409? Please let me know and I can have it sent to you next week.

The suppier of ab409 was able to inform us that Ab409 was not tested on p53 and p65.

We would suggest using BSA in TBST solution. Do not hesite to contact us for any further information.

Yes, ab409 -- Mouse monoclonal (11A1) to N-epsilon acetyl lysine is suitable for use in detecting acetylated histones and other acetylated proteins. Suggested dilution for Western blot is 1/500 to 1/5000.

This antibody should recognise all acetylated histones.

Both antibodies have been found to work well in WB. However, neither have been assessd in a supershift experiment, therefore we can't guarantee results.

All the information we have for this antibody is on the datasheet and at present, this is the only antibody agaisnt acetyl-lysine that we currently have in our product range.

Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely sell samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information about o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"