Anti-N Cadherin (phospho Y860)抗体(ab119752)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y860)
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-N Cadherin (phospho Y860)抗体
参阅全部 N Cadherin 一抗 -
描述
兔多克隆抗体to N Cadherin (phospho Y860) -
宿主
Rabbit -
特异性
Immunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733). -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human N Cadherin (phospho Y860).
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阳性对照
- C2C12 and Human endothelial cells treated with pervanadate.
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常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab119752 was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119752于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa).
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ICC |
1/200.
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说明 |
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WB
1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa). |
ICC
1/200. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
序列相似性
Contains 5 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- Unigene: 464829 Human
- Unigene: 606106 Human
- Unigene: 257437 Mouse
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别名
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
图片
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ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
Detection used an appropriate secondary antibody conjugated to Cy3. -
Lanes 1-2 : Anti-N Cadherin (Cytoplasmic) at 1/1000 dilution
Lanes 3-4 : Anti-N Cadherin
(phospho Y820) at 1/1000 dilution
Lanes 5-6 : Anti-N Cadherin (a.a. 853-864) at 1/1000 dilution
Lanes 7-8 : Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution
Lanes 1 & 5 & 7 : Human endothelial cells, untreated
Lanes 2 & 4 & 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells, untreated
Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Predicted band size: 99 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab119752 被引用在 2 文献中.
- de Souza JM et al. mGluR5 regulates REST/NRSF signaling through N-cadherin/ß-catenin complex in Huntington's disease. Mol Brain 13:118 (2020). PubMed: 32859226
- Xiong Y et al. Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma. Oncotarget 8:24902-24914 (2017). Human . PubMed: 28212546