The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa).
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis. Detection used an appropriate secondary antibody conjugated to Cy3.
Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752)
Lanes 1 & 5 & 7 : Human endothelial cells, untreated Lanes 2 & 4 & 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min Lane 3 : Human endothelial cells, untreated Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 min