Anti-N Cadherin (phospho Y820)抗体(ab76059)

概述

  • 产品名称
    Anti-N Cadherin (phospho Y820)抗体
    参阅全部 N Cadherin 一抗
  • 描述
    兔多克隆抗体to N Cadherin (phospho Y820)
  • 特异性
    ab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phospho-peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N cadherin.

  • 阳性对照
    • Human endothelial cells treated with pervanadate (1 mM) for 30 min.
  • 常规说明
    Do not aliquot this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 100 kDa.
ELISA 1/2000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 序列相似性
    Contains 5 cadherin domains.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

图片

  • All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution

    Lane 1 : Human endothelial cells untreated
    Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
    Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
    Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide


    Predicted band size : 100 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

文献

ab76059 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% triton-x
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (differentiated embryonic stem cell)
Specification
differentiated embryonic stem cell
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme free
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
live cells
Username

Abcam user community

Verified customer

提交于 Jul 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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