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RabMAb

Anti-N Cadherin抗体[EPR1791-4] (ab76011)

概述

  • 产品名称
    Anti-N Cadherin抗体[EPR1791-4]
    参阅全部 N Cadherin 一抗
  • 描述
    兔单克隆抗体[EPR1791-4] to N Cadherin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human N Cadherin aa 150-250 (extracellular). The exact sequence is proprietary.

  • 阳性对照
    • WB: HUVEC, C6 and HeLa cell lysates. IHC-P: Mouse pancreas tissue.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Predicted molecular weight: 100 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能
      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
    • 序列相似性
      Contains 5 cadherin domains.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CADH2_HUMAN antibody
      • Cadherin 2 antibody
      • Cadherin 2 N cadherin neuronal antibody
      • Cadherin 2 type 1 antibody
      • Cadherin 2 type 1 N cadherin neuronal antibody
      • Cadherin 2, type 1, N-cadherin (neuronal) antibody
      • Cadherin-2 antibody
      • Cadherin2 antibody
      • Calcium dependent adhesion protein neuronal antibody
      • CD325 antibody
      • CD325 antigen antibody
      • CDH2 antibody
      • CDHN antibody
      • CDw325 antibody
      • CDw325 antigen antibody
      • N cadherin 1 antibody
      • N-cadherin antibody
      • NCAD antibody
      • Neural cadherin antibody
      • OTTHUMP00000066304 antibody
      • OTTHUMP00000067378 antibody
      see all

    图片

    • Lanes 1-2 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/20000 dilution
      Lane 3 : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/5000 dilution

      Lane 1 : Huvec
      cell lysate
      Lane 2 : C6
      cell lysate
      Lane 3 : HeLa
      cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 100 kDa
      Observed band size: 140 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry of kidney carcinoma staining N Cadherin with ab76011 at 1μg/ml

    • ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    文献

    This product has been referenced in:
    • Fan D  et al. MicroRNA 26b promotes colorectal cancer metastasis by downregulating phosphatase and tensin homolog and wingless-type MMTV integration site family member 5A. Cancer Sci 109:354-362 (2018). WB ; Human . Read more (PubMed: 29160937) »
    • Yamagata M  et al. Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation. Front Mol Neurosci 11:142 (2018). Read more (PubMed: 29760652) »

    See all 18 Publications for this product

    客户评价及客户问答

    Thank you for contacting us and your interest in our products.
    The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

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    Thank you for your reply.

    Can you please confirm what species your samples are from, including cell line or tissue type?

    Also what is the order number for this product? I will need to ensure that the order is not more than 6 months ...

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    Thank you for your reply.

    You're having trouble with the ab76011, anti-N cadherin antibody, correct? Not the ab1791 histone H3? You had included ab76011 in your initial email, but ab1791 in your reply.

    Do you have a lot # from the via...

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    Thank you very much for your interest in Abcam antibodies.

    We have 2 rabbitantibodies directed against the extracellular part of the N-cadherin :

    http://www.abcam.com/ab76011, rabbit monoclonal, tested in Western Blot, ICC/IF, Flow ...

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    Thank you for your enquiry.

    ab76011 is a tissue culture supernatant product. The antibody has not been purified. However, in general products of this type can be presumed to contain around 0.05mg/ml specific antibody.

    I hope this is...

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    Thank you for your enquiry.

    I can confirm that ab76011 antibody is sold as unpurified tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentrati...

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    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital infor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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