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Fusion protein corresponding to N Cadherin (extracellular).
Our Abpromise guarantee covers the use of ab19348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 100 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use a concentration of 5 µg/ml.|
N-cadherin was immunoprecipitated from 300 ug SK-N-SH (human neuroblastoma cell line) whole cell lysate with 5 ug of ab19348. Western blot was performed from the immunoprecipitate using ab19348 at 1/1000 dilution.
Lane 1: ab19348 IP in SK-N-SH whole cell lysate.
Paraffin-embedded human heart tissue stained for N-cadherin using ab19348 at 1/20 dilution in immunohistochemical analysis.
Immunofluorescent analysis of SHSY5Y cells, staining N-Cadherin (green) using ab19348 at 1/100 dilution.
Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes. Samples were incubated with primary antibody for 1 hour at room temperature before incuation with DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red)
Nuclear counterstain: Hoechst 33342 (blue).
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