Anti-N Cadherin抗体[8C11] (ab19348)

概述

  • 产品名称
    Anti-N Cadherin抗体[8C11]
    参阅全部 N Cadherin 一抗
  • 描述
    小鼠单克隆抗体[8C11] to N Cadherin
  • 特异性
    This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.
  • 经测试应用
    适用于: WB, ICC/IF, IP, IHC-Pmore details
  • 种属反应性
    与反应: Rabbit, Hamster, Human, Bird
    不与反应: Mouse, Rat, Cow, Pig
  • 免疫原

    Fusion protein corresponding to N Cadherin (extracellular).

  • 阳性对照
    • A431 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab19348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 100 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.
IHC-P 1/20.

靶标

  • 功能
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 序列相似性
    Contains 5 cadherin domains.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

图片

  • N-cadherin was immunoprecipitated from 300 ug SK-N-SH (human neuroblastoma cell line) whole cell lysate with 5 ug of ab19348. Western blot was performed from the immunoprecipitate using ab19348 at 1/1000 dilution.

    Lane 1: ab19348 IP in SK-N-SH whole cell lysate.

  • Lane 1 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/1000 dilution
    Lanes 2 - 3 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 25 µg per lane.


    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-N Cadherin antibody [8C11] (ab19348) at 1/1000 dilution

    Lane 1 : Mouse brain tissue extract at 30 µg
    Lane 2 : MCF-7 (human breast adenocarcinoma cell line) whole cell lysate

    Developed using the ECL technique
  • Paraffin-embedded human heart tissue stained for N-cadherin using ab19348 at 1/20 dilution in immunohistochemical analysis.

  • Immunofluorescent analysis of SHSY5Y cells, staining N-Cadherin (green) using ab19348 at 1/100 dilution.

    Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes. Samples were incubated with primary antibody for 1 hour at room temperature before incuation with DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red)

    Nuclear counterstain: Hoechst 33342 (blue).

     

  • Immunofluorescence analysis of ARPE-19 cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.

    ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an AlexaFluor®488-conjugated donkey anti-mouse secondary IgG for 60 min.

文献

This product has been referenced in:
  • Qi L  et al. TRIM16 suppresses the progression of prostate tumors by inhibiting the Snail signaling pathway. Int J Mol Med 38:1734-1742 (2016). WB ; Human . Read more (PubMed: 27748839) »
  • Lindén S  et al. Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes. Dalton Trans 44:4994-5003 (2015). Read more (PubMed: 25612290) »

See all 10 Publications for this product

客户评价及客户问答

Thank you for contacting us and your interest in our products.
The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

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Thank you for your inquiry.

The immunogen of ab66025 is the full length protein and to our knowledge this clone was not mapped. We therefore do not know where exactly this antibody binds to within the N Cadherin.

According to Swis...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (Eye)
Loading amount
20 µg
Specification
Eye
Gel Running Conditions
Reduced Denaturing (8% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Jul 07 2009

Thank you for your enquiry. Here is the link to the Clustal W alignment and I will also paste the alignment below. http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20060323-21275762&poll=yes The immunogen of ab19348 wa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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