Anti-N Cadherin抗体[8C11] (ab19348)


  • 产品名称Anti-N Cadherin抗体[8C11]
    参阅全部 N Cadherin 一抗
  • 描述
    小鼠单克隆抗体[8C11] to N Cadherin
  • 特异性This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.
  • 经测试应用适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Rabbit, Hamster, Human, Bird
    不与反应: Mouse, Rat, Cow, Pig
  • 免疫原

    Fusion protein corresponding to N Cadherin (extracellular).

  • 阳性对照
    • A431 cell lysate



Our Abpromise guarantee covers the use of ab19348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 100 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.


  • 功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 序列相似性Contains 5 cadherin domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin antibody [8C11] 图像

  • Lane 1 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/1000 dilution
    Lanes 2 - 3 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 25 µg per lane.

    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of ARPE-19 cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.

    ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an AlexaFluor®488-conjugated donkey anti-mouse secondary IgG for 60 min.

Anti-N Cadherin antibody [8C11] (ab19348)参考文献

This product has been referenced in:
  • Chen X  et al. S100 calcium-binding protein A6 promotes epithelial-mesenchymal transition through ß-catenin in pancreatic cancer cell line. PLoS One 10:e0121319 (2015). WB ; Human . Read more (PubMed: 25799022) »
  • Zhu YT  et al. Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells. J Cell Biol 206:799-811 (2014). Human . Read more (PubMed: 25202030) »

See all 8 Publications for this product

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Thank you for contacting us and your interest in our products.
The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino...

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Thank you for your inquiry.

The immunogen of ab66025 is the full length protein and to our knowledge this clone was not mapped. We therefore do not know where exactly this antibody binds to within the N Cadherin.

According to Swis...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Eye)
Loading amount 20 µg
Specification Eye
Gel Running Conditions Reduced Denaturing (8% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jul 07 2009

Thank you for your enquiry. Here is the link to the Clustal W alignment and I will also paste the alignment below. The immunogen of ab19348 wa...

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