Anti-N Cadherin抗体[5D5] (ab98952)

概述

  • 产品名称Anti-N Cadherin抗体[5D5]
    参阅全部 N Cadherin 一抗
  • 描述
    小鼠单克隆抗体[5D5] to N Cadherin
  • 经测试应用适用于: Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Purified recombinant fragment of Human N Cadherin expressed in E. Coli.

  • 阳性对照
    • A431, NIH-3T3, Hela, C6 and LNCap cells. Human lung cancer, colon cancer, ovarian cancer and mammary cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab98952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 100 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 序列相似性Contains 5 cadherin domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin antibody [5D5] 图像

  • ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  • All lanes : Anti-N Cadherin antibody [5D5] (ab98952) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : NIH-3T3 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : LNCap cell lysate


    Predicted band size : 100 kDa
  • ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

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  • Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.
  • Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye
  • ab98952 staining N Cadherin (orange) in Mouse E15.5 Brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBST) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

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Anti-N Cadherin antibody [5D5] (ab98952)参考文献

This product has been referenced in:
  • Duan Y  et al. Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncol Lett 12:2055-2061 (2016). WB . Read more (PubMed: 27602138) »
  • Gou WF  et al. The role of RhoC in epithelial-to-mesenchymal transition of ovarian carcinoma cells. BMC Cancer 14:477 (2014). WB ; Human . Read more (PubMed: 24986540) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryonic mouse brain coronal cortical section)
Permeabilization Yes - Triton X
Specification embryonic mouse brain coronal cortical section
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. Bhavin Shah

Verified customer

提交于 Oct 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step ZytoChem-Plus HRP Polymer-Kit Blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (NSCLC)
Specification NSCLC
Permeabilization No
Fixative HOPE
Username

Abcam user community

Verified customer

提交于 Apr 13 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Zytomed Systems HRP Polymer Blocking solution as blocking agent for 5 minute(s) · Concentration: 100%
Sample Human Cell (paraffin-embedded lung cancer tissue)
Specification paraffin-embedded lung cancer tissue
Permeabilization No
Fixative HOPE
Username

Abcam user community

Verified customer

提交于 Mar 18 2014


ab98952 is an ascites product. As it is not a purified antibody, an exact concentration has not been determined. In general however, the specific antibody concentration in a product such as this is 0.9 - 9mg/ml.

Thank you for contacting us.

I am pleased to provide the following immunogen information for ab98952 N Cadherin antibody [5D5] :

Immunogen sequence: ##########

I am sorry to confirm that permeabilization was required wh...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching ...

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Thanks for the reply.

I am send you a vial of ab7800, mouse monoclonal to cytokeratin 14. Hopefully you will be able to get the desired staining pattern with this antibody. Your new order number is 1132112.

If there is anything else I...

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Thank you for letting me know.

I am sorry about the continued problems that you have been having with ab98952. I will this information onto the lab.

Based on the information you have provided, I believe the best option is to go ahead ...

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Thank you for your reply.

I am sending you a new vial of ab98952 to try out. Your new order number (for your records only) is *
.

If you have any issues with the new vial, or if there is anything else I can help you with, please l...

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Thank you for contacting Abcam.

I have been in contact with the lab regarding the staining pattern that you are observing whilst using ab98952. Our WB assay shows that the antibody is positive against recombinant protein. DNA sequencing result...

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