Anti-N acetylglucosamine kinase抗体(ab95182)

概述

  • 产品名称Anti-N acetylglucosamine kinase抗体
    参阅全部 N acetylglucosamine kinase 一抗
  • 描述
    兔多克隆抗体to N acetylglucosamine kinase
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residue 50 and 100 of Human N acetylglucosamine kinase (NP_060037.2)

  • 阳性对照
    • Whole cell lysate from HeLa cells, 293T cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab95182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.59 antibody
    • GlcNAc kinase antibody
    • GNK antibody
    • HSA242910 antibody
    • N acetylglucosamine kinase antibody
    • N-acetyl-D-glucosamine kinase antibody
    • N-acetylglucosamine kinase antibody
    • nagK antibody
    • NAGK_HUMAN antibody
    see all

Anti-N acetylglucosamine kinase antibody 图像

  • All lanes : Anti-N acetylglucosamine kinase antibody (ab95182) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa at 50 µg
    Lane 2 : Whole cell lysate from HeLa at 15 µg
    Lane 3 : Whole cell lysate from HeLa at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 37 kDa


    Exposure time : 30 seconds
  • Detection of Human N acetylglucosamine kinase by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) N acetylglucosamine kinase was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 30 seconds.

Anti-N acetylglucosamine kinase antibody (ab95182)参考文献

ab95182 has not yet been referenced specifically in any publications.

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