Anti-Myostatin Propeptide抗体[4B9] (ab37257)

概述

  • 产品名称Anti-Myostatin Propeptide抗体[4B9]
    参阅全部 Myostatin Propeptide 一抗
  • 描述
    小鼠单克隆抗体[4B9] to Myostatin Propeptide
  • 经测试应用适用于: ELISA, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein, corresponding to amino acids 24-266 of Human Myostatin Propeptide (Human myostatin propeptide without signaling sequence). The recombinant protein contains 5 additional amino acid residues: 2 at the N-terminal end (MG) and 2 at the C-termial end (KLN).

  • 阳性对照

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号4B9
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P 1/200.
IHC-Fr 1/50.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Acts specifically as a negative regulator of skeletal muscle growth.
  • 疾病相关Defects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cmpt antibody
    • GDF-8 antibody
    • GDF8_HUMAN antibody
    • Growth/differentiation factor 8 antibody
    • MSLHP antibody
    • MSTN antibody
    • MSTN myostatin antibody
    • Myostatin antibody
    • Myostatin Propeptide antibody
    see all

Anti-Myostatin Propeptide antibody [4B9] 图像

  • ICC/IF image of ab37257 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37257, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Myostatin Propeptide antibody [4B9] (ab37257)参考文献

ab37257 has not yet been referenced specifically in any publications.

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