概述

  • 产品名称Anti-Myosin VIIa抗体
    参阅全部 Myosin VIIa 一抗
  • 描述
    兔多克隆抗体to Myosin VIIa
  • 特异性Detects Myosin VIIa from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.
  • 经测试应用适用于: IHC-P, ICC, IP, WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Myosin VIIa aa 16-31.
    Sequence:

    SGQEFDVPIGAVVKLC


    (Peptide available as ab4996)

  • 阳性对照
    • WB: mouse testes lysate IHC-P: mouse ear

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC 1/50.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Myosin VIIa peptide (ab4996).
ICC/IF Use at an assay dependent concentration. PubMed: 19429027
IHC-Fr Use at an assay dependent concentration. PubMed: 20461409

靶标

  • 相关性Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
  • 细胞定位Cytoplasmic, cytoskeleton
  • 数据库链接
  • 别名
    • Deafness autosomal dominant 11 antibody
    • Deafness autosomal recessive 2 antibody
    • DFNA11 antibody
    • DFNB 2 antibody
    • DFNB2 antibody
    • Myo7a antibody
    • Myosin 7a antibody
    • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
    • Myosin VIIa antibody
    • Myosin, unconventional, family VII, member A antibody
    • MYOVIIA antibody
    • MYU7A antibody
    • NSRD 2 antibody
    • NSRD2 antibody
    • Unconventional myosin VIIa antibody
    • Ush 1B antibody
    • Ush1b antibody
    • Usher syndrome 1B antibody
    see all

Anti-Myosin VIIa antibody 图像

  • Western blot detection of Myosin VIIa in mouse testes tissue extract using ab3481.

  • ab3481 staining primary cell culture of Pig retinal pigment epithelium by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 148 conjugated goat anti-rabbit antibody, diluted 1/750, was used as the secondary.

    See Abreview

  • ab3481 at a 1/500 dilution staining Myosin VIIa in Mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C in 1% goat serum, 0.1% Triton X-100, 1X PBS. Fixed in formalin. Permeabilized using 0.5% Triton X-100. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at 1/500 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview

  • (A) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin.  (B) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with ab3481 as the primary antibody (dilution 1/900) and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

  • (G) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (H) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with rabbit IgG as an isotype control and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

Anti-Myosin VIIa antibody (ab3481)参考文献

This product has been referenced in:
  • Schmitz HM  et al. Kanamycin-furosemide ototoxicity in the mouse cochlea: a 3-dimensional analysis. Otolaryngol Head Neck Surg 150:666-72 (2014). Mouse . Read more (PubMed: 24415490) »
  • Kim HJ  et al. Conditional deletion of pten leads to defects in nerve innervation and neuronal survival in inner ear development. PLoS One 8:e55609 (2013). IHC-P ; Mouse . Read more (PubMed: 23393595) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK II)
Specification MDCK II
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Nov 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Retinal Pigment Epithelium primary cells)
Specification Retinal Pigment Epithelium primary cells
Fixative Formaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Nov 26 2009

Thank you for your enquiry. There is no stated expiration date for this antibody. There is one year expiration date from the date of purchase, however the Abpromise will be still effective for only 120 days after purchase. This antibody is unli...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary cell culture, retinal pigment epithelium)
Specification Primary cell culture, retinal pigment epithelium
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO, chinese hamster ovary cells)
Specification CHO, chinese hamster ovary cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (ear cross sections)
Specification ear cross sections
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Mrs. Chantal Longo-Guess

Verified customer

提交于 Jun 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Primary cell culture, retinal pigment epithelium)
Specification Primary cell culture, retinal pigment epithelium
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TX100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jun 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (Pig RPE cells)
Loading amount 25 µg
Specification Pig RPE cells
Gel Running Conditions Reduced Denaturing (7%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jun 03 2008

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet and we are unaware if the sequence is also conserved in zebrafish.

Thank you for your enquiry. This antibody has not yet been tested for use with immunofluorescence. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift vo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"