RabMAb

Anti-Myosin, smooth muscle heavy chain 1 and 2抗体[EPR5335] (ab124679)

概述

  • 产品名称Anti-Myosin, smooth muscle heavy chain 1 and 2抗体[EPR5335]
    参阅全部 Myosin, smooth muscle heavy chain 1 and 2 一抗
  • 描述
    兔单克隆抗体[EPR5335] to Myosin, smooth muscle heavy chain 1 and 2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Myosin, smooth muscle heavy chain 1 and 2.

  • 阳性对照
    • WB: Human fetal artery, bladder and mouse spleen lysates IHC: Human colon and kidney tissues
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD KD = 1.85 x 10 -10 M Learn more about KD
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR5335
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 250 kDa (predicted molecular weight: 227 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能Muscle contraction.
  • 组织特异性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • 疾病相关Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • 序列相似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 细胞定位Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • KIAA0866 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SM1 antibody
    • SM2 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 antibody
    see all

Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] 图像

  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/20000 dilution (purified) + mouse spleen at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/50000 dilution (purified) + human fetal artery lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

  • Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

  • All lanes : Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)

    Lane 1 : Human fetal artery lysate
    Lane 2 : Human bladder lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)参考文献

This product has been referenced in:
  • Wang D  et al. Local MicroRNA Modulation Using a Novel Anti-miR-21-Eluting Stent Effectively Prevents Experimental In-Stent Restenosis. Arterioscler Thromb Vasc Biol 35:1945-53 (2015). IHC . Read more (PubMed: 26183619) »
  • Castellanos-Rivera RM  et al. Recombination Signal Binding Protein for Ig-?J Region Regulates Juxtaglomerular Cell Phenotype by Activating the Myo-Endocrine Program and Suppressing Ectopic Gene Expression. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24904090) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"