重组Anti-smooth muscle Myosin heavy chain 11抗体[EPR5335] (ab124679)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5335] to smooth muscle Myosin heavy chain 11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-smooth muscle Myosin heavy chain 11抗体[EPR5335]
参阅全部 smooth muscle Myosin heavy chain 11 一抗 -
描述
兔单克隆抗体[EPR5335] to smooth muscle Myosin heavy chain 11 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal artery, bladder and mouse spleen lysates IHC: Human colon and kidney tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.85 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5335 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124679于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 250 kDa (predicted molecular weight: 227 kDa).
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 250 kDa (predicted molecular weight: 227 kDa). |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Muscle contraction. -
组织特异性
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. -
疾病相关
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
序列相似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
结构域
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
细胞定位
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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数据库链接
- Entrez Gene: 4629 Human
- Entrez Gene: 17880 Mouse
- Omim: 160745 Human
- SwissProt: P35749 Human
- SwissProt: O08638 Mouse
- Unigene: 460109 Human
- Unigene: 250705 Mouse
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别名
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
图片
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Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/20000 dilution (purified) + mouse spleen at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 227 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/50000 dilution (purified) + human fetal artery lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 227 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)
Lane 1 : Human fetal artery lysate
Lane 2 : Human bladder lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 227 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab124679 被引用在 10 文献中.
- Jacobsen NL et al. Myofibre injury induces capillary disruption and regeneration of disorganized microvascular networks. J Physiol 600:41-60 (2022). PubMed: 34761825
- Wang F et al. Targeted disruption of the histone lysine 79 methyltransferase Dot1L in nephron progenitors causes congenital renal dysplasia. Epigenetics 16:1235-1250 (2021). PubMed: 33315499
- Sun J et al. Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction. Front Physiol 11:593966 (2020). PubMed: 33424621
- Castillero E et al. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery. Am J Physiol Heart Circ Physiol 315:H1463-H1476 (2018). PubMed: 30141986
- Duivenvoorden HM et al. Discriminating the earliest stages of mammary carcinoma using myoepithelial and proliferative markers. PLoS One 13:e0201370 (2018). PubMed: 30044853
- Zhu ZR et al. MiR-140-3p is Involved in In-Stent Restenosis by Targeting C-Myb and BCL-2 in Peripheral Artery Disease. J Atheroscler Thromb 25:1168-1181 (2018). PubMed: 29760303
- Miller KK et al. Thalidomide treatment prevents chronic graft rejection after aortic transplantation in rats - an experimental study. Transpl Int 30:1181-1189 (2017). PubMed: 28672061
- Krishnan P et al. Enhanced neointimal fibroblast, myofibroblast content and altered extracellular matrix composition: Implications in the progression of human peripheral artery restenosis. Atherosclerosis 251:226-233 (2016). PubMed: 27399649
- Wang D et al. Local MicroRNA Modulation Using a Novel Anti-miR-21-Eluting Stent Effectively Prevents Experimental In-Stent Restenosis. Arterioscler Thromb Vasc Biol 35:1945-53 (2015). IHC . PubMed: 26183619
- Castellanos-Rivera RM et al. Recombination Signal Binding Protein for Ig-?J Region Regulates Juxtaglomerular Cell Phenotype by Activating the Myo-Endocrine Program and Suppressing Ectopic Gene Expression. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24904090