Anti-Myosin Phosphatase (phospho T853)抗体(ab59203)

概述

  • 产品名称
    Anti-Myosin Phosphatase (phospho T853)抗体
    参阅全部 Myosin Phosphatase 一抗
  • 描述
    兔多克隆抗体to Myosin Phosphatase (phospho T853)
  • 特异性
    Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 853 (RSTPGV)

  • 阳性对照
    • Human colon carcinoma. NIH/3T3 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能
    Regulates myosin phosphatase activity.
  • 组织特异性
    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 序列相似性
    Contains 6 ANK repeats.
  • 发展阶段
    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • 结构域
    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻译后修饰
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 细胞定位
    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase (phospho T853) antibody 图像

  • All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunizing phosphopeptide


    Predicted band size : 115 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)参考文献

This product has been referenced in:
  • Cheng CI  et al. The prognostic values of leukocyte rho kinase activity in acute ischemic stroke. Biomed Res Int 2014:214587 (2014). WB ; Human . Read more (PubMed: 24716192) »

See 1 Publication for this product

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Thank you for submitting an Abreview of ab59203. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Left ventricle)
Loading amount
20 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Aug 29 2012

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