Anti-Myosin Phosphatase (phospho T696)抗体(ab59202)

概述

  • 产品名称
    Anti-Myosin Phosphatase (phospho T696)抗体
    参阅全部 Myosin Phosphatase 一抗
  • 描述
    兔多克隆抗体to Myosin Phosphatase (phospho T696)
  • 特异性
    Detects endogenous levels of Myosin Phosphatase only when phosphorylated at threoine 696.
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 696 (RSTPQG)

  • 阳性对照
    • Human breast carcinoma tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能
    Regulates myosin phosphatase activity.
  • 组织特异性
    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 序列相似性
    Contains 6 ANK repeats.
  • 发展阶段
    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • 结构域
    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻译后修饰
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 细胞定位
    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

图片

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

文献

This product has been referenced in:
  • Sastre E  et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . Read more (PubMed: 27484028) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your inquiry.

We don't have a polymer kit besides our mouse on mouse polymer kit, ab127055.

http://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

We have ABC kits and EXPOSE kits.

http:/...

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Thank you for your reply.


This is just to go along with the polymer kit that was used. If you are using polymer detection, simply follow the manufacturers instructions. You can omit this step if not using this type of amplification.
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Thank you for contacting Abcam regarding ab59202.



The IHC protocol is as follows:



Deparaffinization

1. Incubate slide at 60℃ for 60 minutes.

2. Deparaffinize in Xylene for 10 minutes and repeat on...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
120 µg
Specification
Hela
Treatment
10ng/ml IL6 for 30 minutes
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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