Anti-Myosin Phosphatase抗体(ab70809)

概述

  • 产品名称Anti-Myosin Phosphatase抗体
    参阅全部 Myosin Phosphatase 一抗
  • 描述
    兔多克隆抗体to Myosin Phosphatase
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 842) of human Myosin Phosphatase (NP_002471.1)

  • 阳性对照
    • Whole cell lysate from HeLa or 293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Regulates myosin phosphatase activity.
  • 组织特异性Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • 序列相似性Contains 6 ANK repeats.
  • 发展阶段Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • 结构域Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • 翻译后修饰Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • 细胞定位Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase antibody 图像

  • All lanes : Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 115 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • 1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.

Anti-Myosin Phosphatase antibody (ab70809)参考文献

ab70809 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (RT4-D6P2T Rat Schwanoma Cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Specification RT4-D6P2T Rat Schwanoma Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Lars Riecken

Verified customer

提交于 Nov 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"