The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Regulates myosin phosphatase activity.
组织特异性Expressed in striated muscles, specifically in type 2a fibers (at protein level).
序列相似性Contains 6 ANK repeats.
发展阶段Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
结构域Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
翻译后修饰Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
细胞定位Cytoplasm. Along actomyosin filaments and stress fibers.
ab111237, at 3.75 µg/ml, staining Myosin Phosphatase in formalin fixed, paraffin embedded Human skeletal muscle tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
Anti-Myosin Phosphatase antibody (ab111237)参考文献
has not yet been referenced specifically in any publications.