重组
RabMAb

Anti-Myosin light chain kinase抗体[EP1458Y] (ab76092)

概述

  • 产品名称
    Anti-Myosin light chain kinase抗体[EP1458Y]
    参阅全部 Myosin light chain kinase 一抗
  • 描述
    兔单克隆抗体[EP1458Y] to Myosin light chain kinase
  • 经测试应用
    适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminal of human Myosin light chain kinase

  • 阳性对照
    • WB: Human brain lysate IHC-P: Human smooth muscle tissue IF: SH-SY5Y cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EP1458Y
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Predicted molecular weight: 211 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
    • 组织特异性
      Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
    • 序列相似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 fibronectin type-III domain.
      Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      MLCK is probably down-regulated by phosphorylation.
      The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
    • Information by UniProt
    • 数据库链接
    • 别名
      • deglutamylated form antibody
      • DKFZp686I10125 antibody
      • EC 2.7.11.18 antibody
      • FLJ12216 antibody
      • Kinase related protein antibody
      • Kinase-related protein antibody
      • KRP antibody
      • MLCK antibody
      • MLCK1 antibody
      • MLCK108 antibody
      • MLCK210 antibody
      • MSTP083 antibody
      • MYLK antibody
      • MYLK_HUMAN antibody
      • MYLK1 antibody
      • Myosin light chain kinase antibody
      • Myosin light polypeptide kinase antibody
      • OTTHUMP00000180642 antibody
      • OTTHUMP00000180643 antibody
      • smMLCK antibody
      • smooth muscle antibody
      • Smooth muscle myosin light chain kinase antibody
      • Telokin antibody
      see all

    Anti-Myosin light chain kinase antibody [EP1458Y] 图像

    • ab76092 staining Myosin light chain kinase in mouse oocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 overnight at 4°C and blocked with 3% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/50) for 12 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin embedded human smooth muscle, using ab76092, at 1/100 dilution.

    Anti-Myosin light chain kinase antibody [EP1458Y] (ab76092)参考文献

    This product has been referenced in:
    • Zhai L  et al. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS One 12:e0174414 (2017). WB ; Rat . Read more (PubMed: 28388666) »
    • Lin Q  et al. IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI Insight 1:e89402 (2016). WB ; Mouse . Read more (PubMed: 27777977) »

    See all 11 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Embryonic Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Oct 31 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 20 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (colon from Nonalcoholic Steatohepatitis mouse)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.5% typsin
    Specification
    colon from Nonalcoholic Steatohepatitis mouse
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 22 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 16 2015

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% BIS TRIS)
    Loading amount
    20 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 16 2015

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 21 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Oocyte)
    Permeabilization
    Yes - 0.1%triton 4? overnight
    Specification
    Oocyte
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 12 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Sample
    Human Cell (Human arterial myocytes)
    Specification
    Human arterial myocytes
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Apr 08 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8% gel was used)
    Sample
    Rat Tissue lysate - whole (jejunal segment)
    Specification
    jejunal segment
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Dapeng Chen

    Verified customer

    提交于 Sep 13 2013



    The immunogen of ab76092 is specific for MLCK1 and shows no similarity with MLCK2. It was taken from a region that is contained within all isoforms of MLCK1, so it will recognize all of these isoforms.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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