Anti-Myosin light chain 3抗体[MLM520] (ab679)

概述

  • 产品名称Anti-Myosin light chain 3抗体[MLM520]
    参阅全部 Myosin light chain 3 一抗
  • 描述
    小鼠单克隆抗体[MLM520] to Myosin light chain 3
  • 特异性This antibody reacts with ventricle myosin light chain I.
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Cow, Human, Pig
  • 免疫原

    Human ventricle myosin light chain I.

性能

应用

Our Abpromise guarantee covers the use of ab679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Regulatory light chain of myosin. Does not bind calcium.
  • 疾病相关Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
    Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • 序列相似性Contains 3 EF-hand domains.
  • 翻译后修饰The N-terminus is blocked.
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac myosin light chain 1 antibody
    • CMH8 antibody
    • CMLC1 antibody
    • ELC of myosin antibody
    • Essential light chain of myosin antibody
    • MLC1SB antibody
    • MLC1V antibody
    • MYL3 antibody
    • MYL3_HUMAN antibody
    • Myosin light chain 1 antibody
    • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
    • Myosin light chain 1 slow, B antibody
    • Myosin light chain 3 antibody
    • Myosin, light chain 1, ventricular antibody
    • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
    • myosin, light polypeptide 3, alkali antibody
    • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
    • OTTHUMP00000165922 antibody
    • Slow skeletal ventricular myosin alkali light chain 3 antibody
    • slow-twitch muscle B/ventricular isoform antibody
    • Ventricular/slow twitch myosin alkali light chain antibody
    • VLC1 antibody
    see all

Anti-Myosin light chain 3 antibody [MLM520] 图像

  • Procedure:
    Coat microplate with porcine cardiac myosin light chain I.
    Wash 3 times
    Block with 1% BSA-PBS
    Add MLM520 diluted with BSA-PBS
    Incubate for 1 h at room temp.
    Wash 3 times
    Add HRP-labelled anti-mouse IgG diluted with 1% BSA-PBS
    Incubate for 1 h at room temp.
    Wash 3 times
    Add TMBZ solution.
    Incubate few min.
    Measure A450 nm.

  • ab679 (1µg/ml) staining myosin light chain in human heart (left ventricle), using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of cardiomyocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Myosin light chain 3 antibody [MLM520] (ab679)参考文献

This product has been referenced in:
  • Berna M & Ackermann B Increased throughput for low-abundance protein biomarker verification by liquid chromatography/tandem mass spectrometry. Anal Chem 81:3950-6 (2009). ELISA . Read more (PubMed: 19388669) »
  • Berna MJ  et al. Strategic Use of Immunoprecipitation and LC/MS/MS for Trace-Level Protein Quantification: Myosin Light Chain 1, a Biomarker of Cardiac Necrosis. Anal Chem 79:4199-205 (2007). Read more (PubMed: 17447729) »

See all 2 Publications for this product

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